海洋动物细胞培养与组织再生实验室
Laboratory of Cell Culture & Tissue Regeneration of Marine Animals
Team Leader: Prof. Huarong GUO
研究方向

    海洋动物细胞培养方面主要开展以下研究:对虾细胞的体外培养及其基因转移技术研究;鱼类细胞的体外培养与建系以及鱼类和鱼类细胞的体内和体外毒理学研究;珊瑚和砗磲细胞及其共生藻的体外培养与生态系统修复研究。

海洋动物组织再生方面主要围绕组织器官再生的细胞和分子机制以及再生的进化机制这些重要科学问题,开展以下研究:头索动物文昌鱼尾部再生的分子和进化机制;两栖类蝾螈的器官、组织再生过程中的分子和细胞学机制;鱼类鳍的再生过程中的基因调控和细胞外基质重塑机制。



研究队伍


     

 郭华荣 教授,博士生导师

 E-mail: huarongguo@ouc.edu.cn  

 Tel: 18854261970

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 梁宇君 讲师,硕士生导师

 E-mail: liangyujun@ouc.edu.cn  

Tel: 13370819230

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承担课题

·国家自然科学基金项目2项:嗜对虾细胞的高效病毒报告基因表达系统研究及其在转基因对虾上的应用(2015-2018),对虾细胞的分子重编程与永生性转化研究(2012-2015)。

·国家863项目:海洋生物分子细胞遗传育种关键技术(2012-2016)

·厦门市海洋经济创新发展区域示范项目:海洋药源生物种质资源库建设项目(2012-2017)

·山东省优秀中青年科学家科研奖励基金项目:对虾体外培养细胞的永生性转化研究(2012-2014

·青岛市应用基础研究项目:构建转基因鱼类细胞-快速检测污染物遗传毒性新技术(2010-2012



近期论文及成果

·Longjun Pu, Jing Wang, Xiaojuan Zhang, Huarong Guo* (2017). Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cells. Aquaculture, 467, 198-210.

·Cuicui Liang, Yuhao Wang, Yujie Wang, Huarong Guo* (2017). Telomerase reverse transcriptase gene from greasyback shrimp Metapenaeus ensis: isolation, genomic organization and expression pattern analysis.  Zoological Science, 34(5), 419-428.

·Li Han, Ya-Nan Zhai, Yang Liu, Linhua Hao*, Huarong Guo* (2017). Comparison of the In Vitro and In Vivo Toxic Effects of three Sizes of Zinc Oxide (ZnO) Particles using Flounder Gill (FG) Cells and Zebrafish Embryos. Journal of Ocean University of China161),93-106.

·Jie He , Yujun Liang*, Chenghao Xie , Hanchao Qiao, Pao Xu.Expression patterns of the hatching enzyme genes during embryonic development of tilapia.Aquaculture, 2017,479: 845-849.

·Qiongxuan Lu, Tao Liu, Xianming Tang, B0 Dong*, Huarong Guo*(2016). Reformation of tissue balls from tentacle explants of coral Goniopora lobata: self-organization process and response to environmental stresses. In Vitro Cell.Dev.Biol.—Animal, 53(2), 111-122.

·Pathirana A, Diao MY, Huang SB, Zuo LL, Liang Yujun*. Alpha 2 macroglobulin is a maternally-derived immune factor in amphioxus embryos: New evidence for defense roles of maternal immune components in invertebrate chordate. Fish Shellfish Immunol. 2016, 50:21-26.

·Qian Han, Dandan Dong, Xiaojuan Zhang, Cuicui Liang, Qiongxuan Lu, Huarong Guo* (2015). Problems with the use of liposome- and retrovirus-mediated gene transfer methods in the primary lymphoid cells of the Oka organs of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844). Crustaceana, 88 (12-14), 1351-1365.

·Manish Raj Pandey, Huarong Guo*2015. Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos. J. Ocean Univ. China, 14 (2), 362-374.

<!--[if !supportLists]-->·<!--[endif]-->Xu QY, Liang Yujun*. Developmental Expression Patterns of Alpha 2 Macroglobulin in Amphioxus: An Insight into Liver Evolution. Ann Mar Biol Res. 2015, 2(1): 1007.

·Manish Raj Pandey, Huarong Guo*(2014). Evaluation of cytotoxicity, genotoxicity and embryotoxicity of insecticide propoxur using flounder gill (FG) cells and zebrafish embryos. Toxicology in Vitro, 28(3), 340-353.

·Qian Han, Pengtao Li, Xiongbin Lu, Zijuan Guo, Huarong Guo*2013. Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis. Aquaculture 410-411,101-113.

·Deyu Geng, Zhixia Zhang, Huarong Guo*2012. Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression. Biosensors2(3), 318-340.

·郭华荣(2011. 双语教材《Cytotechnology/细胞工程技术》,中国海洋大学出版社.

·Fan Yang, Qianqian Zhang, Huarong Guo*, Shicui Zhang* (2010). Evaluation of cytotoxicity, genotoxicity and teratogenicity of marine sedimentsfrom Qingdao coastal areas using in vitro fish cell assay, comet assayand zebrafish embryo test. Toxicology in Vitro24, 2003-2011.

·Na Na, Huarong Guo*(2009). In vitro and in vivo acute toxicity of fenpyroximate to flounder Paralichthys olivaceus and its gill cell line FG. Aquatic toxicology 92, 76-85.



其他

有关研究成果已获国家发明专利3项:一种对虾细胞培养基(ZL201310162351.8)、一种用于对虾细胞的逆转录病毒基因转移系统(ZL201510192146.5)和一种用于对虾细胞的启动子(ZL201510046128.6),以及实用新型发明专利1项(一种水生动物胚胎细胞收集器,专利号:201420430124.9)。