• 研究方向


    动物胚胎发育和成体器官再生,是通过网络化的分子信号通路精细调控以决定细胞命运的生物学过程。细胞信号转导是其中关键的调控机制。靶细胞通过受体接收信号细胞分泌出来的配体分子,引发其胞内的信号级联反应,最终诱导该细胞向特定命运分化。信号转导途径异常可导致胚胎发育异常、出生缺陷以及多种疾病。对于信号转导途径调控机理的研究有助于深入理解动物胚胎发育过程,并为理解相关人类发育缺陷及疾病的发生机理提供理论依据。

    本实验室以果蝇和斑马鱼为主要模式生物,结合遗传学、蛋白与转录组学、分子生物学等现代技术手段深入探究Hedgehog、Notch、JNK等多种重要发育信号的转导和调控机制,以期阐明这些信号通路在胚胎发育及成体器官(心脏)再生过程中的功能和作用机理。



  • 承担课题


    · 中国海洋大学“筑峰人才工程”启动经费

    · 中国海洋大学“青年英才工程”启动经费

    · 国家自然科学基金面上项目2项,青年项目1项


  • 近期论文及成果


    *通讯作者,#共同第一作者

      

    (一)发育

    1. Wang L, Yang J, Wang H, Ran C, Su Y, Zhao L*. (2019) A highly selective turn-on fluorescent prob detection of Aluminum and its application to bio-imaging. Sensors19(11): 2423.

    2. Chi C, Wang L, Lan W, Zhao L*, Su Y*.(2018) PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing. Apoptosis 23:554-562.

    3. Zhao L, Wang L, Chi C, Lan W, Su Y*. (2017) The emerging roles of phosphatases in Hedgehog pathway. Cell Communication and Signaling 15:35-45.

    4. Liu M, Li Y, Liu A, Li R,Su Y, Du J, Li C, Zhu AJ. (2016) The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development.eLife5: e17200.

    5. Du J, Zhang J, He T, Li Y,Su Y, Tie F, Liu M, Harte P, Zhu AJ. (2016) Stuxnet Facilitates the Degradation of Polycomb Protein during Development. Developmental Cell 37: 507-519.

    6. Geisbrecht ER, Sawant K, Su Y, Liu ZC, Silver DL, Burtscher A, Wang X, Zhu AJ, McDonald JA. (2013) Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration. Developmental Dynamics 242: 414-431.

    7. Zhang J, Liu M, Su Y, Du J, Zhu AJ. (2012) A targeted in vivo RNAi screen reveals deubiquitinases as new regulator of Notch signaling.G3: Genes, Genomes, Genetics2: 1563-1575.

    8. Zheng XF, Yang SY, Han YC, Zhao XY, Zhao L, Tian T, Tong J, Xu P, Xiong C, Meng AM. (2012) Loss of zygotic NUP107 protein causes missing of pharyngeal skeleton and other tissue defects with impaired nuclear pore function in zebrafish embryos. The Journal of Biological Chemistry 287(45): 38254-38264.

    9. Su Y, Ospina JK, Zhang J, Michelson A, Schoen AM and Zhu AJ. (2011) Sequential phosphorylation of Smoothened transduces graded Hedgehog signaling.Science Signaling4:ra43.

    10. Du J, Zhang J, Su Y, Liu M, Ospina JK, Zhu AJ. (2011) in vivo RNAi Screen reveals neddylation genes as novel regulators of Hedgehog signaling. PLoS ONE 6: e24168.

    11. Zhao XY, Zhao L, Tian T, Zhang Y, Tong J, Zheng XF, Meng AM. (2010) Interruption of cenph causes mitotic failure and embryonic death, and its haploinsufficiency suppresses cancer in zebrafish.The Journal of Biological Chemistry 285(36): 27924-27934.

    12. Tian T, Zhao L, Zhao XY, Zhang M, Meng AM. (2009) A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b.Journal of Genetics and Genomics 36(10): 581-589.

    13. Tian T#, Zhao L#, Zhang M, Zhao XY, Meng AM. (2009) Both foxj1a and foxj1b are implicated in left-right    asymmetric development in zebrafish embryos. Biochemical and Biophysical Research Communications 380(3): 537-542.

    14. Zhao L, Zhao XY, Tian T, Lu QL, Nirma S, Wu D, Kuang Z, Zheng XF, Han YC, Yang SY, Zhang CM, Meng AM. (2008) Heart-specific isoform of tropomyosin4 is essential for heartbeat in zebrafish embryos Cardiovascular Research 80(2): 200-208.

    15. Su Y#, Zhang L#, Gao X, Meng F, Wen J, Zhou H, Meng A and Chen Y. (2007) The evolutionally conserved activities of Dapper2 in antagonizing TGFb signaling. FASEB Journal21: 682-690.

    16. Lin X#, Duan X#, Liang Y#, Su Y#, Wrighton KH, Long J, Hu M, Davis CM, Wang J, Brunicardi FC, Shi Y, Chen Y, Meng A, Feng X. (2006) PPM1A functions as a Smad phosphatase to terminate TGFb signaling.Cell125: 915-928.

    17. Zhang L#, Zhou H#, Su Y#, Sun Z, Zhang H, Zhang L, Zhang Y, Ning Y, Chen Y, Meng A. (2004) Zebrafish Dpr2 inhibits mesoderm induction by promoting degradation of Nodal receptors. Science306: 114-7.

    18. Su Y and Meng A. (2002) The expression of gbx-2 during zebrafish embryogenesis. Mechanisms of Development 113: 107-10.

      

    (二)再生

    1. Lan W, Liu S, Zhao L*, Su Y*. (2020) Regulation of Drosophila hematopoiesis in lymph gland: from a developmental signaling point of view. International Journal of Molecular Sciences 21(15): 5246.

    2. Wang L, Yang J, Wang H, Ran C, Su Y, Zhao L*. (2019) A highly selective turn-on fluorescent probe for the detection of Aluminum and its application to bio-imaging. Sensors 19(11): 2423.

    3. Chi C, Wang L, Lan W, Zhao L*,Su Y*.(2018) PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing. Apoptosis 23:554-562.

    4. Zhao L, Wang L, Chi C, Lan W, Su Y*. (2017) The emerging roles of phosphatases in Hedgehog pathway. Cell Communication and Signaling 15:35-45.

    5. Zhao L, Ben-Yair R, Burns CE, Burns CG. (2019) Endocardial Notch signaling promotes cardiomyocyte proliferation in the regenerating zebrafish heart through Wnt pathway antagonism. Cell Reports 26(3): 546-554.

    6. Han P, Bloomekatz J, Ren J, Zhang R, Grinstein JD, Zhao L, Burns CG, Burns CE, Anderson RM, and Chi NC. (2016) Coordinating cardiomyocyte interactions to direct ventricular chamber morphogenesis. Nature 534: 700-704.

    7. Jahangiri L, Sharpe M, Novikov N, González-Rosa JM, Borikova A, Nevis K, Paffett-Lugassy N, Zhao L, Adams M, Guner-Ataman B, Burns CE, Burns CG. (2015) The AP-1 transcription factor component Fosl2 potentiates the rate of myocardial differentiation from the zebrafish second heart field. Development143: 113-122.

    8. Mahmoud A, O’Meara C, Gemberling M, Zhao L, Bryant DM, Zheng RM, Gannon JB, Cai L, Choi WY, Egaczyk GF, Burns CG, Burns CE, Macrae CA, Poss KD, Lee RT. (2015) Nerves regulate cardiomyocyte proliferation and heart regeneration. Developmental Cell34: 387-399.

    9. Harrison MR, Bussmann J, Huang Y, Zhao L, Osorio A, Burns CG, Burns CE, Sucov HM, Siekmann AF and Lien CL. (2015) Chemokine guided angiogenesis directs coronary vasculature formation in zebrafish. Developmental Cell33(4): 442-454.

    10. Jang IH, Lu YF, Zhao L, Wenzel PL, Kume T, Datta SM, Arora N, Guiu J, Lagha M, Kim PG, Schlaeger TM, Zon LI, Gigas A, Burns CE, Daley G. (2015) Notch1 acts via Foxc2 to induce the endothelial to hematopoietic transition during embryonic development. Blood125(9): 1418-1426.

    11. Zhao L, Borikova AL, Ben-Yair R, Guner-Ataman B, Macrae CA, Lee RT, Burns CG, Burns CE. (2014) Notch signaling regulates cardiomyocyte proliferation during zebrafish heart regeneration. PNAS 111(4): 1403-1408.

    12. Deacon DC, Nevis KR, Cashman TJ, Zhou Y, Zhao L, Washko D, Guner-Ataman B, Burns CG, Burns CE. (2010) The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis.Development 137(11): 1887-1896.

    13. Zhao XY, Zhao L, Tian T, Zhang Y, Tong J, Zheng XF, Meng AM. (2010) Interruption of cenph causes mitotic failure and embryonic death, and its haploinsufficiency suppresses cancer in zebrafish.The Journal of Biological Chemistry 285(36): 27924-27934.

    14. Tian T, Zhao L, Zhao XY, Zhang M, Meng AM. (2009) A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b.Journal of Genetics and Genomics 36(10): 581-589.

    15. Tian T#, Zhao L#, Zhang M, Zhao XY, Meng AM. (2009) Both foxj1a and foxj1b are implicated in left-right asymmetric development in zebrafish embryos. Biochemical and Biophysical Research Communications 380(3): 537-542.

    16. Zhao L, Zhao XY, Tian T, Lu QL, Nirma S, Wu D, Kuang Z, Zheng XF, Han YC, Yang SY, Zhang CM, Meng AM. (2008) Heart-specific isoform of tropomyosin4 is essential for heartbeat in zebrafish embryos. Cardiovascular Research 80(2): 200-208.