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海洋生物多样性与进化研究所
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  • 关于IEMBAbout IEMB
    • 研究所简介Introduction
    • 组织框架Framework
    • 教授委员会Professor Committee
    • 研究所成员Staff
  • 研究团队Research Groups
    • 原生动物学团队Protozoology Team
      • 原生动物学研究室Laboratory of Protozoology
      • 染色体生物学实验室Laboratory of Chromosome Biology
      • 系统分类学实验室Laboratory of Systematic Taxonomy
    • 发育生物学团队Developmental Biology Team
      • 进化与发育实验室Laboratory for Evolution & Developmental Biology
      • 纤毛与器官发育实验室Laboratory of Cilia Biology & Organogenesis
      • 发育与再生生物学实验室Laboratory of Developmental & Regenerative Biology
      • 细胞与组织动力学实验室 Laboratory of Cell & Tissue Dynamics
    • 演化生物学团队 Evolutionary Biology Team
      • 形态发生与进化实验室Laboratory for Morphogenesis & Evolution
      • 水生寄生虫学实验室Laboratory of Aquatic Parasitology
      • 进化基因组学实验室 Laboratory of Evolutionary Genomics
      • 海洋化能合成生态系统实验室Chemosynthetic Ecosystems Laboratory
      • 头足类分类与系统演化实验室 Laboratory of Cephalopod Taxonomy & Phylogeny
      • 海洋生物整合组学实验室 Laboratory of Integrative Omics of Marine Organisms
    • 生态与环境生物学团队Ecology & Environmental Biology Team
      • 微生物海洋学实验室 Laboratory of Microbial Oceanography
      • 海洋病毒与微型生物多样性实验室 Laboratory of Viral & Microbial Diversity
      • 环境微生物生态实验室Laboratory of Environmental Microbial Ecology
      • 底栖生物实验室Laboratory of Benthos
    • 海洋生物资源学团队Marine Bioresources Team
      • 海洋药用资源生物学实验室 Laboratory of Marine Medical Resources Biology
      • 可持续生物材料实验室Laboratory of Sustainable Biomaterials
      • 海洋生物被膜实验室Laboratory of Marine Biofilms
    • 细胞生物学团队Cell Biology Team
      • 藻类遗传学实验室Laboratory of Algal Genetics
      • 藻类病理学实验室Laboratory of Algal Pathology
      • 海洋动物细胞培养技术实验室Laboratory of Cell Culture Technology of Marine Animals
      • 再生生物学实验室Laboratory of Regenerative Biology
      • 海洋微生物互作实验室Laboratory of Marine Microbial Interaction
  • 科学研究Research
    • 科研进展New Achievements
    • 发表论文Publications
  • 研究生招生Admissions
    • 招生信息For Prospective Students
    • 导师信息Advisors
      • 博士生导师Ph.D. advisors
      • 硕士生导师Master's advisors
  • 运行管理Management
    • 设备预约Equipment Reservation
    • 实验室安全Laboratory Safety
  • 联系我们Contact Us

研究团队
Research Groups

  • 原生动物学团队
    Protozoology Team
  • 发育生物学团队
    Developmental Biology Team
  • 演化生物学团队
    Evolutionary Biology Team
  • 生态与环境生物学团队
    Ecology & Environmental Biology Team
  • 海洋生物资源学团队
    Marine Bioresources Team
  • 细胞生物学团队
    Cell Biology Team
    • 藻类遗传学实验室
      Laboratory of Algal Genetics
    • 藻类病理学实验室
      Laboratory of Algal Pathology
    • 海洋动物细胞培养技术实验室
      Laboratory of Cell Culture Technology of Marine Animals
    • 再生生物学实验室
      Laboratory of Regenerative Biology
    • 海洋微生物互作实验室
      Laboratory of Marine Microbial Interaction
  • 近期论文及成果
    Laboratory of Cell Culture Technology of Marine AnimalsLaboratory of Cell Culture Technology of Marine Animals

  • 当前位置:首页  研究团队  细胞生物学团队  海洋动物细胞培养技术实验室  近期论文及成果

2024 Jing Wang#, Longjun Pu#,Xiaojuan Zhang,Cuicui Liang, Dandan Dong, Jiantao Guan,Huarong Guo*. Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development in Marsupenaeus japonicus. Journal of Oceanology and Limnology, 42(1), 201-215.

2024 Yiwen Tao#, Jinwu Wang#, Rui Xiao, Qingli Zhang, Huarong Guo*. Development and evaluation of a shrimp virus (IHHNV)-mediated gene transfer and expression system for shrimps. International Journal of Molecular Sciences, 25, 8999.

2024 Yaqi Zhao, Ting Xue, Jinwu Wang, Huarong Guo*. Development of reliable in vitro long-term culture systems for hematopoietic cells of crayfish Cherax quadricarinatus and virus susceptibility assay. Aquaculture, 589 (2024), 741016.

2024 Liu Song, Yifu Xu, Yaqi Zhao, Jinwu Wang, Huarong Guo*. Identification of active shrimp U6 promoters for efficient CRISPR/Cas9 gene editing in penaeid shrimps based on a VP28-pseudotyped baculovirus expression system. Aquaculture 591(2024), 741128.

2024 Ting Xue#, Yue Zhang#, Zhenxin Zhao, Yaru Li, Jinwu Wang, Huarong Guo*. Establishment and characterization of a continuous marine fish fin cell line from Epinephelus fuscoguttatus and viral susceptibility to crustacean CMNV. Journal of Oceanology and Limnology. doi: 10.1007/s00343-024-4054-4.

2023 Liwen Guo, Yaqi Zhao, Huarong Guo*. Long-term in-vitro culture and subculture of the hemocytes of swimming crab (Portunus trituberculatus). Journal of Oceanology and Limnology, 41(5):1918-1939.

2023 Yaqi Zhao, Liwen Guo, Huarong Guo*. Routine development of long-term primary cell culture and finite cell line from the peripheral hemolymph of greasyback shrimp (Metapenaeus ensis) and virus susceptibility. Aquaculture, 563(2023), 739007.

2023 Meiling Sun#, Mingdong Liu#, Hong Shan#, Kang Li#, Peng Wang#, Huarong Guo#, Yaqi Zhao, Rui Wang, Yiwen Tao, Liuyan Yang, Ying Zhang, Xiaoming Su, Yunhui Liu, Chunyang Li, James Lin, Xiu-Lan Chen, Yu-Zhong Zhang*, Qing-Tao Shen*. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection. Science advances, 9(8), eadd2796.

2023 Yiwen Tao, Qingli Zhang, Huarong Guo*. Improvement and applications of adeno-associated virus 2 (AAV-2) system for efficient gene transfer and expression in penaeid shrimp cells. Aquaculture Reports, 32 (2023) :101700.

2023 Yaqi Zhao, Huarong Guo*. A novel medium for long-term primary culture of hemocytes of Metapenaeus ensis. MethodsX, 11 (2023), 102335.

2021 Mengxi Wu, Qian Hu, Yang Zhou, Huarong Guo*. Development of a VP28-pseudotyped baculovirus expression system for efficient gene transfer in penaeid shrimps. Aquaculture, 541: 736741.

2021 Na Li, Liwen Guo, Huarong Guo*. Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus). In Vitro Cellular & Developmental Biology-Animal 57:912-931.

2019 Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo*. The improvement and application of lentivirus-mediated gene transfer and expression system in penaeid shrimp cells. Marine Biotechnology, 21: 9–18.

2019 Jing Wang#, Yuqi Lin#, Xin Song#, Longjun Pu, Guangdong Ji, Guorong Li, Huarong Guo*. Promoter of shrimp translationally controlled tumor protein (TCTP) gene has higher activity than cytomegavirus (CMV) promoter in driving foreign genes to express in primary shrimp cells. Crustaceana 92 (9): 1039-1061.

2024 郭华荣;陶奕文. 一种用于在对虾中递送和表达外源基因的对虾病毒表达系统(专利号:ZL202111656.209.X). 国家发明专利.

2024 郭华荣;薛霆. 一种用于长期培养鱼类肌肉干细胞的培养基(专利号:202211627471.6).国家发明专利.

2024 郭华荣;薛霆. 一种用于海水鱼类肌肉干细胞快速分化的分化诱导培养基(专利号:ZL202211615748.3). 国家发明专利.

2024 郭华荣;薛霆。一种用于淡水鱼类肌肉干细胞快速分化的培养基及其应用(专利号:ZL202211616057.5). 国家发明专利.

2024 Huarong Guo; Xin Song;Yang Zhou. Method for continuous culture of shrimp cells (美国专利号:US 11,873,509). 美国发明专利.

2024 Huarong Guo; Mengxi Wu. Pseudotyped insect baculovirus gene transfer system and pseudotyped baculovirus for shrimps, construction method and use thereof (美国专利号:US 12,110,497). 美国发明专利.

2023 郭华荣;郭莉文. 一种用于螃蟹细胞的长期培养和连续传代的培养基(专利号:ZL202210818244.5). 国家发明专利.

2023 郭华荣;赵亚琦. 一种用于对虾细胞长期培养和连续传代的培养基(专利号:ZL202211127826.5). 国家发明专利.

2021 郭华荣;毋梦茜. 一种用于对虾的假型昆虫杆状病毒基因转移系统、病毒及构建方法和应用(专利号:ZL202010196617.0). 国家发明专利.

2020郭华荣;宋欣;周阳. 一种对虾细胞连续培养方法(专利号:ZL201910622127.X). 国家发明专利.

2019 郭华荣;张晓娟;陈月如. 一种对虾胚胎细胞的分离与培养方法(专利号:ZL201610322759.0). 国家发明专利. 


  • 中国海洋大学
  • 中国海洋大学科技处
  • 中国海洋大学人事处
  • 中国海洋大学研究生院
  • 青岛海洋科学与技术试点国家实验室
  • 国家自然科学基金委员会
  • 国家教育部

地址:青岛市鱼山路5号 中国海洋大学达尔文馆 邮编: 266003Tel/Fax: +86 532 8203 1982

Ocean University of China, Yushan Campus, No. 5 Yushan Road,Qingdao 266003,China,E-mail: zhangc@ouc.edu.cn

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