海洋动物细胞培养方面主要开展以下研究：对虾和螃蟹细胞的体外2D和3D培养与建系研究；对虾及其细胞的高效基因转移技术研究；鱼类细胞的体外培养与建系以及鱼类和鱼类细胞的体内和体外毒理学研究；珊瑚和砗磲细胞及其共生藻的体外培养与生态系统修复研究。

       海洋动物组织再生方面主要围绕组织器官再生以及再生-进化方面的科学问题，以文昌鱼、墨西哥钝口螈及鱼类为材料，研究组织器官再生的细胞和分子机制及再生-进化机制。

· 国家重点研发计划“蓝色粮仓科技创新”专项“重要养殖虾蟹类种质创制与健康苗种繁育”项目: 虾蟹类新种质创制技术平台（2018-2022），参加.

· 国家自然科学基金项目2项：嗜对虾细胞的高效病毒报告基因表达系统研究及其在转基因对虾上的应用（2015-2018），对虾细胞的分子重编程与永生性转化研究（2012-2015），主持.

· 国家863项目：海洋生物分子细胞遗传育种关键技术(2012-2016),参加.

· 厦门市海洋经济创新发展区域示范项目：海洋药源生物种质资源库建设项目(2012-2017).

· 山东省自然科学基金面上项目，文昌鱼尾部再生过程中BMP2/4基因的调控，（2012-2015）.

· 山东省优秀中青年科学家科研奖励基金项目：对虾体外培养细胞的永生性转化研究（2012-2014），主持.

· 青岛市应用基础研究项目：构建转基因鱼类细胞-快速检测污染物遗传毒性新技术（2010-2012），主持.

近期代表性论文及成果（*通讯作者，#共同第一作者）

1. Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo*（2019）. The improvement and application of lentivirus-mediated gene transfer and expression system in penaeid shrimp cells. Marine Biotechnology, 21: 9-18.

2. Jing Wang#, Yuqi Lin#, Xin Song#, Longjun Pu, Guangdong Ji, Guorong Li, Huarong Guo*（2019）. Promoter of shrimp translationally controlled tumor protein (TCTP) gene has higher activity than cytomegavirus (CMV) promoter in driving foreign genes to express in primary shrimp cells. Crustaceana, 92 (9) 1039-1061.

3. Yujun Liang*, Delima Rathnayake, Shibo Huang, Anjalika Pathirana, Qiyu Xu, Shicui Zhang*（2019）. BMP signaling is required for amphioxus tail regeneration. Development, 146(4): dev166017.

4. Peng zhang, Xiaolong Yuan, Yongmei Du, Huaibao Zhang, Guoming Shen, Zhongfeng Zhang, Yujun Liang*, Donglin Zhao*, Kuo Xu* （2019）. Angularly Prenylated Indole Alkaloids with Antimicrobial and Insecticidal Activities from an Endophytic Fungus Fusarium sambucinum TE-6L. J Agric Food Chem, 67(43): 11994-12001.

5. Longjun Pu, Jing Wang, Xiaojuan Zhang, Huarong Guo* (2017). Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cells. Aquaculture, 467, 198-210.

6. Cuicui Liang, Yuhao Wang, Yujie Wang, Huarong Guo* (2017). Telomerase reverse transcriptase gene from greasyback shrimp Metapenaeus ensis: isolation, genomic organization and expression pattern analysis.  Zoological Science, 34(5), 419-428.

7. Li Han, Ya-Nan Zhai, Yang Liu, Linhua Hao*, Huarong Guo* (2017). Comparison of the In Vitro and In Vivo Toxic Effects of three Sizes of Zinc Oxide (ZnO) Particles using Flounder Gill (FG) Cells and Zebrafish Embryos. Journal of Ocean University of China，16（1），93-106.

8. Jie He , Yujun Liang *, Chenghao Xie , Hanchao Qiao, Pao Xu (2017).Expression patterns of the hatching enzyme genes during embryonic development of tilapia.Aquaculture, 479: 845-849.

9. Qiongxuan Lu, Tao Liu, Xianming Tang, B0 Dong*, Huarong Guo*(2016). Reformation of tissue balls from tentacle explants of coral Goniopora lobata: self-organization process and response to environmental stresses. In Vitro Cell.Dev.Biol.—Animal, 53(2), 111-122.

10. Pathirana A, Diao MY, Huang SB, Zuo LL, Liang Yujun*(2016). Alpha 2 macroglobulin is a maternally-derived immune factor in amphioxus embryos: New evidence for defense roles of maternal immune components in invertebrate chordate. Fish Shellfish Immunol., 50:21-26.

11. Qian Han, Dandan Dong, Xiaojuan Zhang, Cuicui Liang, Qiongxuan Lu, Huarong Guo* (2015). Problems with the use of liposome- and retrovirus-mediated gene transfer methods in the primary lymphoid cells of the Oka organs of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844). Crustaceana, 88 (12-14), 1351-1365.

12. Manish Raj Pandey, Huarong Guo*（2015）. Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos. J. Ocean Univ. China, 14 (2), 362-374.

13. Xu QY, Liang Yujun*（2015）. Developmental Expression Patterns of Alpha 2 Macroglobulin in Amphioxus: An Insight into Liver Evolution. Ann Mar Biol Res., 2(1): 1007.

14. Manish Raj Pandey, Huarong Guo*(2014). Evaluation of cytotoxicity, genotoxicity and embryotoxicity of insecticide propoxur using flounder gill (FG) cells and zebrafish embryos. Toxicology in Vitro, 28(3), 340-353.

15. Qian Han, Pengtao Li, Xiongbin Lu, Zijuan Guo, Huarong Guo*（2013）. Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis. Aquaculture 410-411,101-113.

授权国家发明专利5项：

一种对虾细胞培养基（ZL201310162351.8）

一种对虾胚胎细胞的分离与培养方法（201610322759.0）

一种用于对虾细胞的启动子（ZL201510046128.6）

一种用于对虾细胞的病毒启动子（201510718934.3）

一种用于对虾细胞的逆转录病毒基因转移系统（ZL201510192146.5）。

授权实用新型发明专利1项：

一种水生动物胚胎细胞收集器（201420430124.9）。